tert-Butyl (((2R,3S,5R,6R)-5-Azido-6-(((1R,2S,3S,4R,6S)-4,6-diazido-3-(((2S,3R,4S,5S,6R)-4-azido-3,5-dihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)oxy)-2-hydroxycyclohexyl)oxy)-3-hydroxytetrahydro-2H-pyran-2-yl)methyl)carbamate is an intermediate used in the synthesis of O-3-Amino-3-deoxy-α-D-glucopyranosyl-(1→6)-O-[2,6-diamino-2,3,4,6-tetradeoxy-α-D-glycero-hex-4-enopyranosyl-(1→4)]-2-deoxy-D-streptamine (A604395), which is an impurity of Tobramycin (T524000), a single factor antibiotic comprising about 10% of nebramycin, the aminoglycosidic antibiotic complex produced by Streptomyces tenebrarius.