人骨肉瘤细胞
细胞名称：MNNG/HOS Cl #5 [R-1059-D]人骨肉瘤细胞
描述：这株细胞是HOS（ATCC CRL-1543TM）经0.01mcg/ml MNNG（一种致癌的亚硝胺）转化而来。 这株细胞饱和浓度高，在软琼脂中的克隆形成率高，并能在裸鼠中成瘤。
动物种别：人
性别：女
组织来源：骨；骨肉瘤
形态：未知
培养基和添加剂：MEM培养基GIBCO,货号41500034，添加NaHCO3 1.5g/L，Sodium Pyruvate 0.11g/L)，90%；优质胎牛血清，10%。 气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。
供应限制：A。仅供研究之用。
REFERENCE：Rhim JS , et al. Transformation of human cells in culture by N-methyl-N'-nitro-N- nitrosoguanidine. Nature 256: 751-753, 1975. PubMed: 1057046 Rhim JS , et al. Characterization of human cells transformed in vitro by N-methyl-N'- nitro-N-nitrosoguanidine. Int. J. Cancer 19: 505-510, 1977. PubMed: 265298 Zhu X , et al. Cell cycle-dependent modulation of telomerase activity in tumor cells. Proc. Natl. Acad. Sci. USA 93: 6091-6095, 1996. PubMed: 8650224 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
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