胰蛋白酶 来源于牛胰腺(TPCK处理), ≥180 units/mg protein (10,350 BAEE/3,450 USP/NF units/mg protein)
Product Name： Trypsin from bovine pancreas(TPCK Treated)
CAS号:9002-07-7
介绍： Trypsin is a pancreatic serine protease with substrate specificity based upon positively charged lysine and arginine side chains. It is derived from a 34 kDa inactive precursor zymogen, trypsinogen, after enzymatic removal of an N-terminal 6-amino acid leader sequence resulting in the 23.8 kDa trypsin molecule. The optimum pH is 8.0. Trypsin is inhibited by organophosphorus compounds such as diisopropylfluorophosphate and natural inhibitors from pancreas. Soybean, lima bean, and egg white are also sources of natural inhibitors. Trypsin cleaves amide and ester bonds of Arg and Lys. The Aladdin Sequencing Grade Trypsin has been further purified to remove trace contaminating proteases and autolysis products which could interfere in trypsin digestion experiments, and exhibits a single band on PAGE.
用途： Trypsin is a serine protease used to hydrolyze proteins. Trypsin from bovine pancreas has a molecular weight of 23.8 kDa. Trypsins are used for the re-suspension of cells during cell culture and in proteomics research for the digestion of various proteins
贮存： 储存温度2-8℃
敏感性： 易潮湿
生化和生理学机理： Trypsin cleaves peptide chains, amides and esters mainly at the carboxyl side of the amino acids lysine or arginine. Trypsin originates in the pancreas in the form of the zymogen trypsinogen.
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